The Chemical Synthesis of Insulin
We have developed an efficient insulin synthesis method that enabled us to synthesise insulin with an overall yield of 20%. The yield was calculated based on the first amino acid of the A-chain. The insulin content of the final product was determined spectrophotometrically. Analytical HPLC and mass spectrometry comparison of synthetic insulin (Synpel Chemical) and recombinant insulin (Merck) showed complete identity (see pictures below).
This method currently enables stable production of insulin analogues in quantities ranging from a few milligrams to several grams. It can be used to develop pilot-scale technology for producing insulin analogues that cannot be produced recombinantly but have high clinical potential.
A complete list of insulin-like peptides and further information is available on our website (www.synpel.cz). We believe our expertise in insulin-like peptides synthesis will be of interest to a wide range of researchers and pharmaceutical companies.
